Microbial Field Test Kit

Peter Maher - The Man Behind Liquid Intelligence

Peter Maher – The Man Behind Liquid Intelligence

You can call Peter now on 1800 441 163 (7am to 7pm seven days a week – including public holidays – except Christmas Day) for a chat about product and application information or to simply purchase Liquid Intelligence.


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“How You Can Detect Algae, Bacteria, Yeast And Mould Contamination In Your Diesel Fuel”

Simply Using The Liquid Intelligence Microbial Field Test Kit

These Products Come With A Technician On Tap

With my special guarantee you have everything to gain and nothing to lose… it’s that easy… and our free technical support can advise you every step of the way… like having a technician on tap.

We understand that everybody’s application can be unique or out of the ordinary. To be sure of the best result call Peter on 1800 441 163 (7am to 7pm seven days a week – including public holidays – except Christmas Day – including public holidays – except Christmas Day) and have a chat about your particular application.


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If you have biologically contaminated diesel fuel (or think you might have) then you are in the right place.

Contamination is:

  • Costly
  • Unproductive
  • A Nuisance.

Diesel Bug Test Kits Whiteboard Video Explanation


Full Product Support

If you need assistance to eradicate an algae, bacteria or mould contamination in your diesel fuel tank, contact our Technical Support Team by phone:1800 441 163 (7am to 7pm seven days a week – including public holidays – except Christmas Day)


Most Diesel Engine Failures Originate In The Fuel Tank

When water is present and the environment hits the right temperature range… dormant algae, fungi, yeast and bacteria can rapidly grow in the area of fuel/water interface at the bottom of the diesel fuel tank.

Microscopic in size…. a single cell, can grow to a biomass of kilograms in just 12 hours.

Symptoms of contamination can range from…. clogged and slimy filters…dark, hazy fuel…..sludge build up in tanks….excess smoke and foul odours. Eventually microbial contamination can clog fuel lines and stop engines….often with critical and expensive consequences to the fuel delivery system.

Fuel tanks and lines may have to be removed and steam cleaned… fuel pump and injectors can also become clogged and may also need to be serviced… this can cost thousands of dollars.

The Liquid Intelligence Diesel Fuel Microbial Field Test Kit can detect the presence of microscopic algae, fungi, yeast and bacteria before its had a chance to develop… allowing sufficient time to take appropriate action to prevent further contamination. For more information about the test kit call Peter Maher on 1800 441 163 (7am to 7pm 7 days a week)


Liquid Intelligence Microbial Field Test Kits Are Recommended For:

Boats - Liquid Intelligence 201 Bacteria And Fungi BiocideDiesel Generators - Liquid Intelligence 201 Bacteria And Fungi BiocideConstruction Equipment - Liquid Intelligence 201 Bacteria And Fungi Biocide
Road Vehicles - Liquid Intelligence 201 Bacteria And Fungi BiocideDiesel Storage Tanks - Liquid Intelligence 201 Bacteria And Fungi BiocideMining Equipment - Liquid Intelligence 201 Bacteria And Fungi Biocide


How Does The Test Kit Work?

The test is easy to use and effective:

  • Liquid Intelligence Diesel Fuel Dip-slide Test Kits use a combination double sided slide for the detection of algae, bacteria, yeasts and moulds in diesel fuel… which provides a simple means of detecting micro-organisms in diesel fuel tanks.
  • The dip slide itself is a simple agar double sided slide contained in a plastic sterile test tube.
  • The lighter colour side of the dip-slide comprises a nutrient agar for growing bacteria cultures.
  • The darker coloured side is comprised of a formulated agar that will grow yeast and mould cultures.
  • Yeast appears as low round white or grey discs, mould appear as furry colonies.


Liquid Intelligence microbial field test kit

Small Bottle

The kit comes in a small (approx 6 inch) plastic container. This incubator-like quality allows the bugs to grow faster when testing.

Microbial Test Kit Slide Liquid Intelligence Diesel Biocide

Remove Slide

Simply remove the slide from the test kit… with fingers touching the handle only.

Microbial Beaker Sample Liquid Intelligence Diesel Biocide

Dip Slide

Dip the slide into the diesel fuel sample for ten seconds

Microbial Field Test Kit After 24hrs Liquid Intelligence Diesel Biocide

Check In Twenty Four Hours

And after twenty four hours the slide will detect any contamination in a diesel fuel tank.

Yeast appears as low round white or grey discs

Mould appears as furry colonies

Mould appears as furry colonies

Bacteria appears as red dots

Bacteria appears as red dots

Microbial Field Test Kit Results Liquid Intelligence Diesel Biocide


How To Interpret The Results

  • Compare the slide surface against the comparison charts printed on the side of the black storage container.
  • Any reaction requires immediate action.
  • Bacteria and fungi will form insoluble particulate matter that can clog fuel filters, resulting in fuel starving and engine stoppage. They can also corrode metal surfaces, including storage tanks and pumps, and will form organic acids that contribute to fuel instability.
  • Liquid Intelligence has a range of broad spectrum biocide treatments for killing algae and bacteria in diesel fuels.


Microbial Field Test Instructions

  1. Obtain diesel sample from the bottom of the fuel tank or from the fuel filter or water separator.
  2. Remove the double sided slide from black storage container. Be careful not to touch the slide surfaces.
  3. Immerse the test slide into the fuel sample for ten seconds, ensuring that both sides of slide are fully coated.
  4. Drain excess fuel from test slide by touching the slide tip on a clean paper towel.
  5. Replace the slide into black storage container and tighten cap. (Bacteria, yeast and mould grow best in the dark.)
  6. Place the black storage container in a warm place (30°C to 35°C) for at least 24 hours. One side of the slide is white. This will detect BACTERIA between 24 and 36 hours after exposure. The other side of the slide is off white. This will detect MOULD and YEAST between 36 and 48 hours after exposure. Note that higher temperatures may inhibit bacterial growth. In the case of negative results, incubation for a further five days can be carried out to detect any slow growing strains. After incubation, the slide surfaces are simply compared against the chart to obtain the contamination level of the bacteria, yeast or mould in your diesel fuel.


How Did Your Biological Contamination Happen?


Biomass Bug Liquid Intelligence Diesel Biocide

Twenty Species

There are known to be over twenty species of fuel bugs that can contaminate a diesel fuel storage tank.

Bacteria In A Beaker Liquid Intelligence Diesel Biocide

Doubling Every Twenty Minutes

Once in a fuel supply they can double their numbers every twenty minutes and one single spore can produce over 260,000 cloned descendants in six hours

Diesel/Water In Tank Liquid Intelligence Diesel Biocide

First It Grows At The Diesel/Water Interface.

The fuel bug grows in the diesel/water interface at the bottom of the fuel tank

Contaminated Engine & Fuel Filters Liquid Intelligence Diesel Biocide

Now Its In Your Engine.

The contamination can be drawn into the engine… quickly clogging fuel filters… eventually leading to engine breakdown. Now it can become expensive.

How Do You Get The Diesel Bug (Algae, Bacteria, Yeast and Fungi)?

Diesel Bug (Bacteria Contamination of Diesel)

Bacteria in diesel is a well known problem to anyone who works with diesel engines, so what is this bug and why does it contaminate diesel?
Diesel is an organic fuel so it provides an ideal environment for microscopic fungi, yeast and bacteria to feed and grow.

This environment provides:

  • Dissolved water for germination
  • Carbon for food
  • Oxygen and sulphur for respiration
  • Trace elements for growth and propagation.

As many as twenty varieties of bacteria are responsible for the majority of problems with diesel engines and their performance. There are many differing types of bacteria which can infect systems and form bio-films on steel surfaces. Accelerated corrosion can also occur wherever the bio-film settles, usually in pits or crevices. Unlike general corrosion, it is an attack on a very specific area.

It is very difficult to determine when a system is first contaminated, but once contaminated diesel enters the fuel system, it is very difficult to eradicate.

Diesel bug can originate from the air or moisture, or during tank filling and/or expansion and contraction of storage tanks, the bacteria cover themselves in a protective film (slime) to protect against biocides and can lie dormant in the minute crevices of the metal, rubber and polyurethane coatings of the fuel tanks and fuel systems.

Then, when water is present and the environment hits the right temperature range, they begin reproduction in the area of fuel/water interface.

Microscopic in size, they can develop into a mat easily visible to the naked eye very rapidly. A single cell, weighing only one millionth of a gram can grow to a biomass of 10 kilograms in just 12 hours, resulting in a biomass several centimetres thick across the fuel/water interface.


Each species has its own characteristics:

And unfortunately…

All of these can and do cause damage to the fuel system.


Bacteria utilise hydrocarbons and reproduce asexually by binary fission; swelling in size as they feed, they then separate into two cells. In this way, microbes double their numbers every 20 minutes, one spore converting to 262,144 in 6 hours.


SRB’s are a specific group of bacteria utilizing simple carbon, not hydrocarbons, and require the activity of other microbes in a consortium. Aerobic (in the presence of oxygen) or anaerobic (without oxygen) bacteria have a combined effect. The aerobic bacteria (sulphate oxidizing) create a film to consume the oxygen first. This allows the anaerobic (sulphate reducing) bacteria to thrive.
SRB’s reduce sulphates and produce hydrogen sulphide. They are directly involved with many microbial corrosion reactions and can cause sulphide souring of stored distillate products. Their action changes the pH creating an acidic environment, conducive to accelerated corrosion. They attach themselves to the steel as a film and go to work. They derive their nutrition from the surrounding environment and multiply. They are particularly difficult to deal with and produce a sludgy by-product with a strong sulphur odour similar to rotten eggs (hydrogen sulphide).


These also contribute to corrosion, eating steel and reducing ferrite to an oxide through a chemical reaction.


Yeasts prefer acidic environments, such as produced by SRB’s. They bud on the parent cell, eventually separating. Reproduction takes several hours.


Fungi grow in the form of branched hypha (a part of the vegetative portion of a fungus that resembles threads), a few microns in diameter, forming thick, tough, intertwined mycelia mats at fuel/water interfaces.


Indeed If You Have Fungal Contamination Of Diesel Fuel On Your Boat… Here’s How You Can Deal With It.

Of all the users of diesel fuel the operators of boats are the most likely to encounter problems with fungus and bacteria for the following reasons:-

Boats operate in a wet environment and it is not always possible stop water seeping into fuel tanks.

The fuel tanks on boats are designed to fit into restricted or unusually shaped areas to make the best possible use of available space. This can mean that they can be difficult to drain and often have areas where pools of water can collect and sit undisturbed.

The fuel tanks contain baffles to stop the fuel sloshing about. The baffles can trap free water in small pockets.

In warmer climates boats operate in areas with high humidity. Water in the air enters the boat tanks through the breather and condenses inside the tanks. Where water is present in pockets in the fuel tanks it will provide a site for fungus and bacteria to grow. Often the fungus or bacteria will develop for a long time without causing any problems until one day they become disturbed and stirred up into the fuel.

This will be noticed when filters start blocking with a black slime which is the dead matter from the fungus and bacteria.

The fungus can be stirred up by :- Vigorous movement due to a storm, etc.

The fungus population reaches a size at which it grows out of its sheltered corner and spreads into the rest of the fuel.

The natural life cycle of the fungus reaches a point at which dieback occurs and the dead matter starts floating in the fuel.

Natural surfactants produced by the fungus cause water and dirt to be suspended in the fuel and the fuel becomes hazy.

PREVENTION On land the normal prevention of fungal problems is to regularly drain any water in which the fungus can grow. This may not be possible in some boats because the fuel tank shape does not provide a common draining point for all water pockets.

An alternative is to consider a regular dosage of Liquid Intelligence 201 Diesel Fuel Bacteria & Fungi Biocide Treatment. The treat rate for a maintenance dose is 1 litre to 2000 litres and to kill a contamination the treat rate is 1 litre to 1000 litres. Tanks treated with a dose of the biocide at every load will stop the fungus growing, regular maintenance should be carried out to remove the water.

TREATMENT Should a tank become over infested with fungus then filters will start blocking with a black slimy deposit and the fuel may be hazy with water and dirt suspended in it. In such cases the biocide should be added to the fuel tank and then the fuel in the tank should be circulated to ensure that the biocide spreads throughout the fuel. Following treatment, the water and dead fungus will drop to the bottom of the tank. It should then be drained off or sucked out with a hose, otherwise it will sit on the bottom until becoming stirred up in rough water and again block filters. Treatment should continue for a few loads until filter operation has returned to normal.



Two Year Money-Back Satisfaction Guarantee

In appreciation for your business, and to demonstrate our confidence in this product, your purchase of the Liquid Intelligence now comes with something extra. In addition to the standard product liability warranty on all Liquid Intelligence products, we now offer a 100% Satisfaction Guarantee.

We want you to be completely satisfied with any Liquid Intelligence product you buy. The Liquid Intelligence 100% Satisfaction Guarantee is our ongoing promise to you that Liquid Intelligence will do whatever it takes to make sure that you are totally satisfied with what you buy from us for two years from the date of purchase.

Liquid Intelligence is the only manufacturer in the business with enough confidence in our products to offer you this unique purchase protection, letting you buy risk free!

How can I make such an outrageous guarantee? Quite simply because I know that Liquid Intelligence works.

It has worked for me, and it has worked for thousands of other customers. And I know that it can work for you.

Quite simply . . . you get to be the judge, jury and executioner if I’m wrong.

Fair Enough?


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In our Sydney office it is now:

We are open 7am to 7pm seven days a week – including public holidays – except Christmas Day Sydney time.
1800 441 163


How Do You get It?

Order by Phone:

Australian Inquiries: 1800 441 163 (7am to 7pm seven days a week – including public holidays – except Christmas Day)
To call from outside Australia please call the number closest to you:
London: (020) 7048 6434
Auckland: (09) 951 5895
Israel: (+97233741371)
South Africa: (+27213001950)
Bahrain: (+97316195134)

Overseas Inquiries: +61 281882060

1_blue_buttonTell us about your diesel fuel problem.
2_blue_buttonWe will discuss your problem and solution with you and we will recommend the best product for your situation.
3_blue_buttonPurchase is made over the phone and usually ships same day by courier, and yes we do ship internationally.
Peter Maher - The Man Behind Liquid Intelligence

Peter Maher – The Man Behind Liquid Intelligence

You can call Peter now on 1800 441 163 (7am to 7pm 7 seven days a week – including public holidays – except Christmas Day) for a chat about product and application information or to simply purchase Liquid Intelligence.

Order by FAX or Mail:

To fax or mail your order, print out this order form and send it to us.

Liquid Intelligence Pty Ltd
Unit 8 / 12 Eddie Road
Minchinbury NSW 2770
Free Call 1800 441 163 (7am to 7pm seven days a week – including public holidays – except Christmas Day)
Fax: (02) 9625 3177

Send Me An Email:

If you would like to know more about the technical aspects of these products and how it relates to your particular vehicle... please send me an email and I will usually respond on the same business day.

Peter Maher

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Peter Maher - The Man Behind Liquid Intelligence Written by Peter Maher

Peter Maher, has been in the chemical industry since he was 18. So that’s more than 40 years he’s been blending, manufacturing and formulating functional automotive and industrial fluids. You can also find Peter on Google+


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