Diesel Fuel Bacteria & Fungi Biocide Treatments

“I Guarantee You Too Can Stop Bacteria, Fungus and Yeast Contamination In Your Diesel Fuel Tank Now, Once and For All… With Liquid Intelligence Diesel Fuel Biocide”

It Really Does Do Exactly What It Says
Like No Other Product Can

Two Year Money-BackSatisfaction Guarantee

Diesel Bug Needs To Be Eradicated Urgently… Here’s Why

Because Its Can Spread So Fast
Watch This Amazing Video To See How Fast It Spreads In Just A Few Minutes

Shown Live In Real Time – Recorded On An Electronic Microscope Camera at 100x Magnification

Video Transcript:

The bacterial culture showcased in this intriguing video has originated from a diesel fuel sample extracted from the bottom of a half full storage tank with suspected symptoms of diesel bug contamination.

In a controlled laboratory setting, we subjected the fuel sample to incubation over a period of 48 hours using a petri dish containing a nutrient-rich agar medium.

Upon close examination at 100 times magnification, the footage captured in real-time video, vividly reveals the robust nature of this particular virulent microbial diesel bug group, highlighting their impressive ability to multiply rapidly.

Amazingly, these types of microbial cells, weighing just one millionth of a gram, have potential to grow into a substantial biomass within a mere 24 hours. As you can see in this video, this exponential growth happens swiftly. Therefore, it would only require a short period for a significant biofilm to develop in the diesel fuel storage tank when given the right conditions as they currently exist in this petri dish sample.

There are about 60 fungus and yeast genetic variations and almost as many bacterial mutations that have been identified in contaminated diesel. Interestingly, all these microorganisms are airborne. They exist in the water molecules suspended in the air. It is from the water that these organisms extract enough oxygen to thrive. And because fuel tanks must be vented, this creates a pathway for air and microbes to flow in and out of a diesel tank.

Even slight changes in temperature can cause air to be drawn into and expelled out of a tank. Diesel tanks provide an ideal environment for microbes due to the heavier density of water compared to diesel. Condensation water collects and settles at the tank’s bottom, forming what is known as ‘water bottom’. Serving as an interface between water and fuel, the water bottom allows microbes to thrive by extracting necessary oxygen from the water below and obtaining energy in the form of carbon and nutrients from the diesel above.

When the fuel in a diesel tank warms up due to engines fuel return flow or from high ambient air temperature, microbe growth can be remarkably swift. Given time, the bacterial infection in the diesel fuel tank showcased in this video, will produce significant volumes of biofilm with thicknesses measured in many centimetres.

And following instances of tank turbulence or movement, fragments of the biofilm, like this sample can detach, posing a risk of obstructing fuel filters and causing fuel supply blockages to the engine. Additionally, some microbes can release acidic substances, which can lead to corrosion in metal fuel tanks and engine fuel delivery components.

This type of bacterial contamination often starts small and unseen in the bottom of diesel fuel tanks. And doesn’t necessarily cause a problem if the fuel is used and replaced regularly. However, vessels, vehicles, equipment or storage diesel fuel tanks that get used infrequently could easily develop a serious diesel bug infection given the right conditions. And serious contamination can cost thousands to fix and will completely take any equipment out of operation.

If you suspect your diesel fuel is biologically contaminated or have concerns in that regard, our initial recommendation is to contact our technical support. Liquid Intelligence offers the most advanced range of diesel fuel bacteria fungi biocides available in the market today. Our qualified technical staff, with over 40 years of experience in the industry, provide personalized assistance. We are accessible from 7 am to 7 pm seven days a week to ensure prompt and expert support tailored to your needs.

“Anyone can now test for diesel bug in the field like a professional in a laboratory. When using Liquid Intelligence Diesel Microbial Field Test Kit.”

Click The Green Button Below
Liquid Intelligence Diesel Microbial Field Test Kit.

Most Diesel Engine Failures Originate In The Fuel Tank

When water is present and the environment hits the right temperature range… dormant fungi, yeast and bacteria can rapidly grow in the area of fuel/water interface at the bottom of the diesel fuel tank.

Microscopic in size…. a single cell, can grow to a biomass of kilograms in just 12 hours.

Symptoms of contamination can range from…. clogged and slimy filters…dark, hazy fuel…..sludge build up in tanks….excess smoke and foul odours. Eventually microbial contamination can clog fuel lines and stop engines….often with critical and expensive consequences to the fuel delivery system.

Fuel tanks and lines may have to be removed and steam cleaned… fuel pump and injectors can also become clogged and may also need to be serviced… this can cost thousands of dollars.

The Liquid Intelligence Diesel Fuel Microbial Field Test Kit can detect the presence of microscopic fungi, yeast and bacteria before its had a chance to develop… allowing sufficient time to take appropriate action to prevent further contamination.

Fungal Contamination Of Diesel Fuel On Your Boat

Of all the users of diesel fuel the operators of boats are the most likely to encounter problems with fungus and bacteria for the following reasons:-

Boats operate in a wet environment and it is not always possible stop water seeping into fuel tanks.

The fuel tanks on boats are designed to fit into restricted or unusually shaped areas to make the best possible use of available space. This can mean that they can be difficult to drain and often have areas where pools of water can collect and sit undisturbed.

The fuel tanks contain baffles to stop the fuel sloshing about. The baffles can trap free water in small pockets.

In warmer climates boats operate in areas with high humidity. Water in the air enters the boat tanks through the breather and condenses inside the tanks. Where water is present in pockets in the fuel tanks it will provide a site for fungus and bacteria to grow. Often the fungus or bacteria will develop for a long time without causing any problems until one day they become disturbed and stirred up into the fuel.

This will be noticed when filters start blocking with a black slime which is the dead matter from the fungus and bacteria.

The fungus can be stirred up by :- Vigorous movement due to a storm, etc.

The fungus population reaches a size at which it grows out of its sheltered corner and spreads into the rest of the fuel.

The natural life cycle of the fungus reaches a point at which dieback occurs and the dead matter starts floating in the fuel.

Natural surfactants produced by the fungus cause water and dirt to be suspended in the fuel and the fuel becomes hazy.

PREVENTION On land the normal prevention of fungal problems is to regularly drain any water in which the fungus can grow. This may not be possible in some boats because the fuel tank shape does not provide a common draining point for all water pockets.

An alternative is to consider a regular dosage of Liquid Intelligence 201 Diesel Fuel Bacteria & Fungi Biocide Treatment. The treat rate for a maintenance dose is 1 litre to 2000 litres and to kill a contamination the treat rate is 1 litre to 1000 litres. Tanks treated with a dose of the biocide at every load will stop the fungus growing, regular maintenance should be carried out to remove the water.

TREATMENT Should a tank become over infested with fungus then filters will start blocking with a black slimy deposit and the fuel may be hazy with water and dirt suspended in it. In such cases the biocide should be added to the fuel tank and then the fuel in the tank should be circulated to ensure that the biocide spreads throughout the fuel. Following treatment, the water and dead fungus will drop to the bottom of the tank. It should then be drained off or sucked out with a hose, otherwise it will sit on the bottom until becoming stirred up in rough water and again block filters. Treatment should continue for a few loads until filter operation has returned to normal.

How Did Your Biological Contamination Happen?

Twenty Species

There are known to be over twenty species of fuel bugs that can contaminate a diesel fuel storage tank.

Doubling Every Twenty Minutes

Once in a fuel supply they can double their numbers every twenty minutes and one single spore can produce over 260,000 cloned descendants in six hours

First It Grows At The Diesel/Water Interface.

The fuel bug grows in the diesel/water interface at the bottom of the fuel tank

Now Its In Your Engine.

The contamination can be drawn into the engine… quickly clogging fuel filters… eventually leading to engine breakdown. Now it can become expensive.

Each species has its own characteristics:

And unfortunately…

All of these can and do cause damage to the fuel system.

BACTERIA

Bacteria utilise hydrocarbons and reproduce asexually by binary fission; swelling in size as they feed, they then separate into two cells. In this way, microbes double their numbers every 20 minutes, one spore converting to 262,144 in 6 hours.

SULPHATE REDUCING BACTERIA (SRB)

SRB’s are a specific group of bacteria utilizing simple carbon, not hydrocarbons, and require the activity of other microbes in a consortium. Aerobic (in the presence of oxygen) or anaerobic (without oxygen) bacteria have a combined effect. The aerobic bacteria (sulphate oxidizing) create a film to consume the oxygen first. This allows the anaerobic (sulphate reducing) bacteria to thrive.

SRB’s reduce sulphates and produce hydrogen sulphide. They are directly involved with many microbial corrosion reactions and can cause sulphide souring of stored distillate products. Their action changes the pH creating an acidic environment, conducive to accelerated corrosion. They attach themselves to the steel as a film and go to work. They derive their nutrition from the surrounding environment and multiply. They are particularly difficult to deal with and produce a sludgy by-product with a strong sulphur odour similar to rotten eggs (hydrogen sulphide).

IRON REDUCING BACTERIA

These also contribute to corrosion, eating steel and reducing ferrite to an oxide through a chemical reaction.

YEASTS

Yeasts prefer acidic environments, such as produced by SRB’s. They bud on the parent cell, eventually separating. Reproduction takes several hours.

FUNGUS

Fungi grow in the form of branched hypha (a part of the vegetative portion of a fungus that resembles threads), a few microns in diameter, forming thick, tough, intertwined mycelia mats at fuel/water interfaces.

Absidia Fungi

The Robert Williams Case Study

This is an example of a Liquid Intelligence Microbial Field Test Kit agar dip slide that has detected bacteria, yeast and fungi in a diesel fuel sample.

The sample was sent to us by a Robert Williams a property manager from north west NSW. He had become concerned that 6000 litres of diesel in his storage tank, was slowly becoming darker. He first noticed this happening soon after taking a delivery from a local bulk fuel supplier. He suspected that the recent delivery was somehow contaminated.

We tested the diesel sample for him using our Microbial Field Test Kit and took these images under a microscope at 12 hour intervals. The fuel sample was taken from a drain tap at the very bottom of the tank and the time laps images indicate that it was contaminated with various strains of microorganisms.

Rob used our Liquid Intelligence 201 Diesel Fuel Biocide to eradicate this infestation before it became a problem. He also purchased some more dip slides so he could check that the bacterial infection was completely eliminated after he had used the Liquid Intelligence 201.

We also suggested that Rob add a dose of Liquid Intelligence 201 Diesel Fuel Biocide into all the equipment that had been filled from the infected tank to insure that the bug did not spread, as a precautionary safeguard.

Rob’s problem is very common, the most likely scenario was that the bacterial contamination was already festering in water at the bottom of his storage tank. And it may have been there undisturbed and undetected for some time. This can be the case when the draw off tap is well above the bottom of the bulk storage tank. And the recent delivery of fuel being pumped into the tank could have stirred up the bottom and dispersed the microorganisms and fouling the diesel.

When water is present from a build-up of condensation dormant algae, fungi, yeast and bacteria can rapidly grow in the area of fuel/water interface at the bottom of the diesel fuel tank. Even though microscopic in size, a single cell, can grow to a biomass of kilograms in days or weeks if left untreated.

Symptoms of contamination can range from…. clogged and slimy fuel filters, dark or hazy fuel, sludge build up in the bottom of fuel tanks and excessive combustion smoke with foul odours. Eventually microbial contamination can clog fuel lines and stop engines….often with critical and expensive consequences to the fuel delivery system.

Storing Diesel Fuel Without A Biocide Is Like Playing Russian Roulette… Here’s Why

Australia now depends almost entirely on imported diesel fuel. Singapore currently supplies a great deal of our diesel fuel needs. And as bacterium, yeast and fungus microorganisms are found absolutely everywhere, and since they are ever-present, it’s easy for them to enter diesel fuel during transport and storage. Particularly when imported diesel is shipped, transported, pumped and stored numerous times from the Asian refineries to the user in Australia. Each freight and storage movement multiplies the risk of the fuel becoming contaminated with bacterium, yeast and fungus microorganisms.

The microorganisms that thrive in diesel fuel are generally aerobic, meaning that they require oxygen, which they can get from the condensation water at the bottom of the fuel tanks. Diesel fuel tanks are always subject to water build-up from condensation. When a fuel tank is warm, the air expands and is forced out. As the tank cools at night, humid air is sucked back into the tank and it’s moisture then condenses on the cooler tank walls and collects as water in the bottom of the tank.

And microbes are of course microscopic, that means that even the smallest amount of water in the bottom of a fuel tank, is enough to help microbiological diesel fuel contamination to occur and colonise. So, most fuel storage tanks will have at least some bacterium growing in them.

As these microorganisms develop, hot conditions can lead them to growing faster. When you factor in humidity as well, the risks of diesel fuel contamination gets higher in regions closer to the equator. So any imported diesel fuel from Asia will carry a greater risk of it already being contaminated with some bacterium, yeast or fungus when you purchase it.

Our Liquid Intelligence 205 Microbial Field Test Kit can detect bacterial contamination BEFORE it has a chance to develop.

This type of bacterial contamination often starts small and unseen in the bottom of a fuel tank. And doesn’t necessarily cause a problem if the fuel is used within a few months. However vessels, vehicles, equipment or bulk diesel fuel tanks that get used infrequently could easily develop a serious diesel bug infection in time. And serious contamination can cost thousands to fix and will completely take any equipment out of operation. But early detection of a diesel bug could allow the fuel to be treated using Liquid Intelligence 201 Diesel Fuel Bacteria & Fungi Biocide. This treatment would sterilise the diesel and fuel tanks, eradicating the bacterium and preventing that potential costly equipment down time.

Two Year Money-Back Satisfaction Guarantee

Two Year Money-Back Satisfaction Guarantee

In appreciation for your business, and to demonstrate our confidence in this product, your purchase of Liquid Intelligence now comes with something extra. In addition to the standard product liability warranty on all Liquid Intelligence products, we now offer a 100% Satisfaction Guarantee.

We want you to be completely satisfied with any Liquid Intelligence product you buy. The Liquid Intelligence 100% Satisfaction Guarantee is our ongoing promise to you that Liquid Intelligence will do whatever it takes to make sure that you are totally satisfied with what you buy from us for two years from the date of purchase.

Liquid Intelligence is the only manufacturer in the business with enough confidence in our products to offer you this unique purchase protection, letting you buy risk free!

How can I make such an outrageous guarantee? Quite simply because I know that Liquid Intelligence works.

It has worked for me, and it has worked for thousands of other customers. And I know that it can work for you.

Quite simply . . . you get to be the judge, jury and executioner if I’m wrong.

Fair Enough?

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This web cam photo of Peter Maher’s Minchinbury warehouse office is updated periodically during the day.

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Simply specify the product and quantities and your full address (including country) and I can usually reply with a quotation within one to two hours during our Sydney, Australia office hours (7am to 7pm seven days per week) with a full quote including delivery as well as a payment link so you can easily pay online using Paypal or a credit card.

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Alternatively, you can call us on +61 2 8188 2060 and get a fast quote over the phone.

 
Regards, Peter Maher

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Peter Maher, has been in the chemical industry since he was 18. So that’s more than 40 years he’s been blending, manufacturing and formulating functional automotive and industrial fluids.

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